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Isolation and identification of strain degrading aflatoxin B1 and its application in peanut meal
  
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KeyWord:aflatoxin B1  degradation  Bacillus amyloliquefaciens  peanut meal
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XU Mingqian  
CAI Guolin  
ZHU Dewei，etc  
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Abstract:
      A few strains potentially degrading aflatoxin B1（AFB1） were primarily screened with the medium using coumarin as the sole carbon source,then the object strain effectively degrading AFB1 was obtained by combining solid state fermentation experiment for degrading AFB1 with Aspergillus flavus inhibition experiment. The strain was identified as Bacillus amyloliquefaciens through morphological observation and sequence alignment of 16S rDNA. The strain could not only degrade 8823% of AFB1 from peanut meal with low AFB1 content （12.32 μg/kg ）, but also degrade 8139% of AFB1 from peanut meal with high AFB1 content （145.17 μg/kg ）, and the content of AFB1 in peanut meal was below 30 μg/kg after degradation, which was in accordance with the national limitation requirement of AFB1 in feed（≤50 μg/kg）.
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