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大豆7S和11S蛋白中氨基酸组成与表面疏水性的相关性研究
Correlation between amino acid composition and surface hydrophobicity of soybean 7S and 11S proteins
  
DOI：
中文关键词:  纯品7S和11S蛋白  Superdex 200凝胶柱层析  SDS-PAGE  氨基酸  表面疏水性
英文关键词:pure 7S and 11S proteins  Superdex 200 gel column chromatography  SDS-PAGE  amino acid  surface hydrophobicity
基金项目:
Author NameAffiliation
LI Dan  
JIANG Lianzhou  
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中文摘要:
      以6个具有代表性的大豆品种为实验材料提取7S和11S蛋白，经Superdex 200凝胶柱层析纯化制得纯品7S和11S蛋白，用SDS-PAGE和蛋白质纯化仪凝胶柱层析两种方法验证其纯度在90%以上。研究了7S和11S蛋白中氨基酸组成与表面疏水性的相关性。结果表明：7S和11S蛋白的表面疏水性与甘氨酸、脯氨酸、胱氨酸/半胱氨酸、丙氨酸、缬氨酸、蛋氨酸、酪氨酸、天冬氨酸、苏氨酸、组氨酸的含量呈正相关；与异亮氨酸、亮氨酸、苯丙氨酸、丝氨酸、赖氨酸的含量呈负相关；与谷氨酸和精氨酸的含量不相关。疏水性氨基酸总量与7S和11S蛋白的表面疏水性呈正相关，亲水性氨基酸总量与其表面疏水性不相关。
英文摘要:
      The 7S and 11S proteins were extracted from six representative soybean varieties. The purities of 7S and 11S proteins which were purified by the Superdex 200 gel column chromatography and validated by SDS-PAGE and protein purification device gel column chromatography were more than 90%. The correlation between amino acid composition and surface hydrophobicity of 7S and 11S proteins was studied. The results showed that the surface hydrophobicity of 7S and 11S proteins was positively correlated with the contents of Gly, Cys, Pro, Ala, Val, Met, Tyr, Asp, Thr and His, while it had negative correlation with the contents of Ile, Leu, Phe, Ser and Lys, and it was not related with the contents of Glu and Arg. It was indicated that the content of hydrophobic amino acids was positively correlated with the surface hydrophobicity of 7S and 11S proteins, while the content of hydrophilic amino acids was not associated with its surface hydrophobicity.
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