|
| Determination of four polycyclic aromatic hydrocarbons in edible oil by solid-phase extraction coupled with high performance liquid chromatography |
| |
| DOI： |
| KeyWord:edible oil polycyclic aromatic hydro carbons (PAHs) determination solid-phase extraction high performance liquid chromatogra phy-fluorescence detection |
| FundProject: |
|
| Hits: 1189 |
| Download times: 2 |
| Abstract: |
| A rapid method for the determination of four polycyclic aromatic hydrocarbons (PAHs)（Chr,BaA,BbF and BaP） in edible oil was developed by solid-phase extraction coupled with high performance liquid chromatography-fluorescence detection (HPLC-FLD).The solid-phase extraction column was self-made and the solid-phase extraction conditions were optimized.The results showed that the edible oil sample could be analyzed directly by solid-phase extraction after it was diluted in isopropanol. The optimal solid-phase extraction conditions were obtained as follows：1.0 g Benzo Ⅱ SPE，with 8 mL acetone as desorption solvent，4 mL isopropanol as washing solvent，2 mL 0.2 g/mL isopropanol diluted oil. The linearity range，correlation coefficient，the limit of detection and relative standard deviation of the proposed method were 1-50 ng/mL(2-50 ng/mL for BbF)，0.999 2-0.999 8，0.14-0.76 ng/g and 2.7%-9.9%.The relative error between determined value and reference value of BaP in three kinds of olive oils provided by National Institute of Metrology in China was 10.3%. The established method had the items of short time,small consumption of organic solvent and simpleness，which was suitable for the determi nation of four PAHs in edible oils. |
| View full text View/Add Comment Download reader |
| Close |
