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| Enzymatic synthesis of dodecanoic acid xylose ester in non-aqueous media |
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| KeyWord:xylose dodecanoic acid dodecanoic acid xylose ester esterification |
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| Abstract: |
| Dodecanoic acid xylose ester was synthesized by xylose and dodecanoic acid. The effects of solvent species，molar ratio of xylose to dodecanoic acid，dosage of molecular sieve，dosage of enzyme and reaction time on the esterification rate were investigated. Then the preliminary separation and identification of the reaction product were conducted by thin layer chromatography（TLC），silica gel column chromatography，HPLC and FTIR. The results showed that the esterification rate of dodecanoic acid could reach 98.70％ under the conditions of reaction time 24 h，reaction temperature 55℃,dosage of enzyme 0.4%，dosage of molecular sieve 40 g/L and molar ratio of xylose to dodecanoic acid 1∶2. The Rf values of xylose and dodecanoic acid xylose ester were 0.75 and 0.63 respectively when developing agent was methanol-water（volume ratio 7∶3）. The analysis results of HPLC and FTIR indicated the formation of ester bond in the reaction product. |
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