| The relative contents of phosphatidylcholine(PC), lysophosphatide (LPC)and glycerylphosphorylcholine (GPC)obtained from the hydrolysis of soy lecithin by phospholipase A1 were determined by proton nuclear magnetic resonance spectroscopy. The peaks with chemical shift at 4.40-4.50, 4.14-4.16 and 3.76-3.83 were chosen as the characteristic proton peaks of PC, LPC and GPC respectively, and their relative contents were calculated by the correspondance between the relative areas of proton peak and amount of substance. The results showed that the recoveries of standard addition and the relative standard deviations (RSD) of PC, LPC and GPC were 101.6%-107.0%, 79.8%-92.4% and 102.0%-107.1%; 1.6%-2.5%,3.6%-6.5% and 5.6%-6.7%, respectively. The RSD of PC, LPC and GPC in the precision experiment were 4.2%, 3.5% and 2.3% respectively. The relative contents of PC, LPC and GPC obtained from the hydrolysis of soy lecithin by phospholipase A1 for 4 h and 8 h were determined by the established method as 71.2%, 22.1% and 6.7%; 22.4%, 0 and 77.6%, respectively. |
