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Optimization of aqueous enzymatic extraction of adlay bran oil by response surface methodology
  
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KeyWord:adlay bran oil  aqueous enzymatic extraction  response surface methodology  fatty acid
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Author NameAffiliation
LIN Li  
QIN Likang  
LIU Ronglin，etc  
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Abstract:
      Using adlay bran as raw material，the process conditions of aqueous enzymatic extraction of adlay bran oil were optimized by response surface methodology. The optimal extraction conditions were obtained by regression equation model as follows：ratio of solid to liquid 1∶6，enzymolysis pH 6，enzymolysis temperature 50℃，enzymolysis time 3 h，dosage of enzyme 0.8% （0.8% α-amylase and 0.8% neutral protease）. And the average yield of adlay bran oil was 76.61% through three parallel verification experiments. Besides, it was found that adlay bran oil had 9 kinds of fatty acids，in which the content of unsaturated fatty acids was over 85%.
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