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Saio法提取大豆7S球蛋白的纯化及鉴定
Purification and identification of soybean 7S globulin extracted by Saio method
  
DOI：
中文关键词:  大豆  7S球蛋白  纯化  鉴定
英文关键词:soybean  7S globulin  purification  identification
基金项目:
Author NameAffiliation
HUANG Xingjian  
ZHANG Zhaomin  
YANG Fang，etc  
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中文摘要:
      研究利用Sephadex G-200凝胶柱层析法和纤维素DE-52离子交换柱层析法分离纯化经过Saio法提取的大豆7S球蛋白样品，采用SDS-聚丙烯酰胺凝胶电泳和高效毛细管电泳进一步鉴定Saio法提取的大豆7S球蛋白样品的组分及含量。结果表明，Saio法提取的大豆7S球蛋白纯度约为81%。
英文摘要:
      The soybean 7S globulin extracted by Saio method was separated and purified by the methods of Sephadex G-200 gel chromatography and cellulose DE-52 ion-exchange chromatography, and its component and content were further identified by SDS-PAGE and high performance capillary electrophoresis. The results showed that the purity of soybean 7S globulin extracted by Saio method was about 81%.
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