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| 溶血磷脂酰胆碱酰基转移的调控机理研究 |
| Regulation mechanism of acyl migration of lysophosphatidylcholine |
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| DOI： |
| 中文关键词: 溶血磷脂酰胆碱 酰基转移 方向性 调控机理 |
| 英文关键词:lysophosphatidylcholine acyl migration direction regulatory mechanism |
| 基金项目: |
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| 中文摘要: |
| 通过对磷脂酶A1（Lecitase Ultra）酶解磷脂酰胆碱（PC）过程进行分析，证实了Sn-2-溶血磷脂酰胆碱（Sn-2-LPC）存在酰基转移现象。依据前人对单甘酯自动酰基转移机理的研究，推测了Sn-2-LPC在酸、碱条件下自动酰基转移的机理，并利用HPLC-RID确定了Sn-2-LPC可以酰基转移生成Sn-1-LPC，而Sn-1-LPC不存在酰基转移现象。同时，以硼酸为例，推测Al(OH)3、Zn(OH)2、B(OH)3 3种两性氢氧化物抑制Sn-2-LPC酰基转移的机理，确定了3种抑制剂的最小添加量分别为0.97、0.85、0.72 mg/mL。为合成酰基磷脂混合物，以及解释产生混合酰基磷脂的生物合成途径（溶血磷脂中间体）提供了理论基础和数据支撑。 |
| 英文摘要: |
| Acyl migration phenomenon of Sn-2-lysophosphatidylcholine(Sn-2-LPC) was confirmed through hydrolysis process analysis of phosphatidylcholine by phospholipase A1(Lecitase Ultra). Based on previous studies on the automatical acyl migration mechanism of monoglyceride, the automatical acyl migration mechanism of Sn-2-LPC in the acid and alkali conditions was speculated. Acyl migration directionalities of Sn-2-LPC and Sn-1-LPC were explored by HPLC-RID. It was found that acyl migration of Sn-2-LPC to Sn-1-LPC happened, while acyl migration of Sn-1-LPC was not present. Meanwhile, the inhibition mechanism of acyl migration of Sn-2-LPC through Al(OH)3, Zn(OH)2 and B(OH)3 was speculated by boric acid. The minimum dosages of Al(OH)3, Zn(OH)2 and B(OH)3 were determined as 0.97, 0.85 mg/mL and 0.72 mg/mL. A theoretical basis and data support for the synthesis of mixed acyl phospholipids and the explanation of biosynthetic pathway (lysophospholipid intermediate) of mixed acyl phospholipids were provided. |
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