| The lipid productions of Trichosporon sp.JM-B under different nutrition and fermentation conditions were compared by flask fermentation. With cell biomass, lipid productivity, lipid content and lipid coefficient as indexes, the composition of fermentation medium including mass concentration of carbon source, composition and mass concentration of nitrogen source and mass concentration of phosphate, and fermentation conditions including inoculation amount,fermentation temperature, shaker speed and fermentation time were optimized, and the fatty acid composition of the lipid was determined by gas chromatography. The results showed that the optimal composition of fermentation medium was obtained as follows: glucose 100 g/L, ammonium sulfate 1.0 g/L, yeast extract 10 g/L, potassium dihydrogen phosphate 4 g/L, natural pH; the optimal fermentation conditions were obtained as follows: fermentation temperature 25℃, shaker speed 160 r/min,seedage of seed liquid 36 h, inoculation amount 10%(based on the volume of broth)and fermentation time 144 h; under the optimal conditions, the cell biomass and lipid productivity were the highest, reaching 16.14 g/L and 6.64 g/L, respectively; compared with the lipid obtained under the initial culture conditions, the lipid productivity under the optimal culture conditions improved by 161.4%, while the content of saturated fatty acids in the lipid decreased by 28.1%.Therefore,Trichosporon sp.JM-B could be considered as a strain with great potential for producing lipid. |
