| Based on GB/T 22110—2008, a simple and rapid method for the determination of squalene in algal oil by gas chromatography-mass spectrometry(GC-MS) was developed.The effect of dosage of methyl esterification reagent on the degree of esterification was investigated, and the impact of different chromatographic columns on separation degree of squalene was also studied. After 0.2 g algal oil was methyl esterified by 1 mL of 0.5 mol/L potassium hydroxide-methanol solution and extracted by hexane, it was separated by HP-5MS chromatographic column, then the squalene was determined quantitatively by GC-MS in selected ion monitoring mode. The linear range, the relative coefficient, the limit of detection and the recovery of standard addition of the method were 10-500 mg/L, 0.999 8, 0.10 mg/L, 92.14%-96.47%, respectively. The method also could be applied to other vegetable oils. By comparing the difference between “complex” and “non-complex” algal oils in composition and content, it was found that in addition to the different contents of fatty acid in these two types of algal oils, the content of squalene in “non-complex” algal oil was 10 times higher than “complex” algal oil. |
