| Cottonseed 7S and 12S globulins were isolated and purified from cottonseed storage protein by protein purification system coupled with gel chromatography,and the protein contents, amino acid compositions, denaturation temperatures and fluorescence intensities of 7S and 12S globulins obtained were determined and analyzed. The results showed that the protein contents of cottonseed 7S and 12S globulins were （91.59±0.53）% and （93.35±0.62）% respectively, and there was no impurity band existed in the SDS-PAGE chromatography of cottonseed 7S and 12S globulins except for the characteristic bands; cottonseed 7S and 12S globulins were rich in Glu, Asp and Arg, and poor in Cys and Met, which was similar to that of most oil seeds; the differential scanning calorimetry（DSC）results indicated that the denaturation temperatures of cottonseed 7S and 12S globulins were 93.3℃ and 107.7℃, respectively; and the maximum fluorescence intensities of cottonseed 7S and 12S globulins were both at about 325 nm. |
