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Optimization of fermentation process for production of DHA oil by Schizochytrium sp.
  
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KeyWord:Schizochytrium sp.  DHA  fermentation  process optimization
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LI Li  
DOU Guangpeng  
HUO Wenyan，etc  
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Abstract:
      The accumulation of DHA oil produced by Schizochytrium sp.was real-timely monitored by phosphoric acid vanillin method, and the fermentation process for production of DHA oil by Schizochytriumsp.was optimized. The results showed that the optimal formular of culture medium for growth and oil accumulation of Schizochytrium sp. were obtained as follows: glucose 30 g/L, corn steep powder 6 g/L, peptone 4 g/L, sodium nitrate 3.6-3.9 g/L, sea crystal 15 g/L. A certain dosage of glucose was fed to increase carbon-nitrogen ratio to improve oil accumulation and a fed batch of 3.0 g/L soybean oil could stimulate cell growth in 50 L fermentor. After fed batch fermentation for 72 h, wet weight of cell reached 200 g/L,total oil content was more than 60% and DHA content in the fatty acid composition of the oil accounted for about 22%.
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