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| 响应面法优化亚麻胶微波 - 超声波辅助提取工艺 |
| Optimization of microwave-ultrasound-assisted extraction of flaxseed gum by response surface methodology |
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| DOI： |
| 中文关键词: 亚麻胶 响应面法 微波-超声波辅助提取 FTIR |
| 英文关键词:flaxseed gum response surface methodology microwave-ultrasound-assisted extraction FTIR |
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| 中文摘要: |
| 为提高亚麻胶提取速率和得率，用微波-超声波辅助提取亚麻胶，采用响应面法优化亚麻胶的提取工艺条件,并利用FTIR分析了亚麻胶的组成。以液料比、提取温度和提取时间为影响因素，亚麻胶得率为响应值，在单因素试验的基础上，通过Box-Behnken试验，建立亚麻胶得率的二次多项式回归方程，经响应面回归分析得到优化组合条件。结果表明，最佳提取工艺条件为提取温度85 ℃、液料比17.3∶1、总提取时间65 min。在最佳条件下，亚麻胶得率为5.14%，与理论值5.33%接近。FTIR分析表明，亚麻胶主要由多糖和蛋白质组成。 |
| 英文摘要: |
| Response surface methodology was applied to optimize the microwave-ultrasound-assisted extraction conditions of flaxseed gum so as to improve the extraction rate and yield of flaxseed gum. Meanwhile, the composition of flaxseed gum was analysed by FTIR. Using ratio of liquid to material, extraction temperature and extraction time as influence factors,and yield of flaxseed gum as response value,the quadratic polynomial regression equation was established by the Box-Behnken design on the basis of single factor experiment. The results showed that the optimal extraction conditions of flaxseed gum were obtained by response surface methodology as follows: extraction temperature 85 ℃, ratio of liquid to material 17.3∶1 and total extraction time 65 min. Under these conditions, the yield of flaxseed gum was 5.14%, closed to the theoretical value (5.33%). The FTIR result indicated that flaxseed gum was mainly composed of polysaccharides and protein. |
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