| The extraction and purification conditions of Eucommia ulmoides seed protein were optimized by single factor experiment and orthogonal experiment,and the amino acid composition and content ofEucommia ulmoides seed protein were analyzed. The results showed that the optimal extraction conditions were obtained as follows: extraction time 50 min, extraction temperature 45℃, ratio of solid to liquid 1∶20,extraction times twice, stirring speed 40 r/min, dosage of sodium bisulfite 0.25%, acid precipitation pH 3.9; and the optimal purification conditions were obtained as follows: washing times 3 times, volume fraction of ethanol 85%, ratio of solid to liquid 1∶6, washing temperature -10℃, washing time 30 min;under the optimal conditions, the extraction rate and purification yield of Eucommia ulmoides seed protein were 85.6% and 97.6%, respectively. 18 amino acids were detected fromEucommia ulmoides seed protein,in which the variety of essential amino acid was complete and the content was high. |
