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溶血磷脂酰乙醇胺硅胶柱色谱纯化技术研究 |
Purification of lysophosphatidylethanolamine using silica gel column chromatography |
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DOI: |
中文关键词: 磷脂酰乙醇胺 溶血磷脂酰乙醇胺 硅胶柱色谱 纯化 |
英文关键词:phosphatidylethanolamine lysophosphatidylethanolamine silica gel column chromatography purification |
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中文摘要: |
探讨了溶血磷脂酰乙醇胺(LPE)硅胶柱色谱纯化的可行性。通过选择,确定以硅胶为固定相、氯仿-甲醇(体积比2∶1)为洗脱液,进行LPE纯化工艺优化。得到的最优工艺条件为:上样量1∶[KG-2mm]40(样品质量与固定相质量比),上样液质量浓度30 mg/mL,洗脱流速2.5 mL/min;在最佳工艺条件下,最终LPE产品纯度为99.05%,回收率为88.69%。研究为进一步探讨工业化制备LPE提供了基础数据和理论支撑。 |
英文摘要: |
The feasibility of purification of lysophosphatidylethanolamine (LPE) using silica gel column chromatography was studied. Through selection, choosing silica gel as stationary phase and chloroform- methanol(volume ratio 2∶1)as eluent, the purification process of LPE was optimized as follows: sample amount 1∶40 (mass ratio of sample to stationary phase), mass concentration of sample 30 mg/mL, flow rate of eluent 2.5 mL/min; under these conditions,the purity and recovery rate of LPE were 99.05% and 88.69%,respectively. The study provided fundamental data and theoretical support for the further industrial preparation of LPE. |
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