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| 菜籽蛋白和酚的固-液相平衡 |
| Solid-liquid phase equilibrium of rapeseed protein and phenols |
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| DOI： |
| 中文关键词: 菜籽蛋白 酚 相互作用 固-液相平衡 分配系 |
| 英文关键词:rapeseed protein phenols interaction solid-liquid phase equilibrium partition coefficient |
| 基金项目: |
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| 中文摘要: |
| 为探索消除菜籽蛋白中酚污染的技术途径，采用菜籽蛋白沉淀率和菜籽蛋白-酚分配系数为指标，考察了菜籽蛋白与酚共存体系的固-液相平衡关系，并基于相行为特征提出了菜籽蛋白提取工艺的改进措施。结果表明：在碱性环境下，酚酸和单宁均可与菜籽蛋白发生共沉淀作用，且浓度越高该作用越显著；在pH 4环境下，菜籽蛋白不仅溶解度最低，而且与单宁或菜籽多酚的结合作用最弱；而在溶液中添加1%的NaCl有利于进一步提升菜籽蛋白和酚的分离效果。在菜籽蛋白的富集或提取工艺中，溶剂用量（液固比）、pH以及盐添加量都可对菜籽蛋白和酚的相互作用产生影响，对这些参数的优化将有利于提高菜籽蛋白提取的选择性并降低酚的混杂。 |
| 英文摘要: |
| With precipitation rate of rapeseed protein and partition coefficient of rapeseed protein-phenols as indexes, the solid-liquid phase equilibrium of coexistence system of rapeseed protein and phenols was studied so as to explore approaches for eliminating the phenols contamination in rapeseed protein, and the improvement measures of extraction process of rapeseed protein was proposed based on the phase behavior characteristics. The results showed that in the alkaline environment, the coprecipitation of rapeseed protein with tannin or rapeseed polyphenols occurred and became more significant with concentration increasing; rapeseed protein showed the lowest solubility and its interactions with tannin or rapeseed polyphenols were the weakest at pH 4; the separation effect of rapeseed protein and phenols was further improved when 1% NaCl was added. Solvent dosage（ratio of liquid to solid）, pH and NaCl dosage had effects on the interactions between rapeseed protein and phenols in the extraction or enrichment process of rapeseed protein, and the optimization of these parameters was beneficial to enhance the selectivity of rapeseed protein extraction and reduce the contamination of phenols. |
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