With precipitation rate of rapeseed protein and partition coefficient of rapeseed protein-phenols as indexes, the solid-liquid phase equilibrium of coexistence system of rapeseed protein and phenols was studied so as to explore approaches for eliminating the phenols contamination in rapeseed protein, and the improvement measures of extraction process of rapeseed protein was proposed based on the phase behavior characteristics. The results showed that in the alkaline environment, the coprecipitation of rapeseed protein with tannin or rapeseed polyphenols occurred and became more significant with concentration increasing; rapeseed protein showed the lowest solubility and its interactions with tannin or rapeseed polyphenols were the weakest at pH 4; the separation effect of rapeseed protein and phenols was further improved when 1% NaCl was added. Solvent dosage(ratio of liquid to solid), pH and NaCl dosage had effects on the interactions between rapeseed protein and phenols in the extraction or enrichment process of rapeseed protein, and the optimization of these parameters was beneficial to enhance the selectivity of rapeseed protein extraction and reduce the contamination of phenols. |