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| 基于代谢调控深黄被孢霉产γ-亚麻酸培养基优化 |
| Optimization of medium for the production of γ-linolenic acid by Mortierella isabellina based on metabolic regulation |
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| DOI： |
| 中文关键词: 深黄被孢霉γ-亚麻酸 代谢调控 Plackett-Burman实验 响应面法 培养基优化 |
| 英文关键词:Mortierella isabellina γ-linolenic acid metabolic regulation Plackett-Burman experiment response surface methodology medium optimization |
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| 中文摘要: |
| 研究深黄被孢霉产γ-亚麻酸过程的代谢调控，从中得出对深黄被孢霉产γ-亚麻酸有促进作用的营养成分，并利用Plackett-Burman实验对培养基营养成分进行筛选。得出葡萄糖、KNO3、乙酸钠、柠檬酸钠为影响产油量的关键因素。在此基础上，以γ-亚麻酸产量为响应值，采用响应面法优化，确定4个关键因素的最佳水平。综合Plackett-Burman实验和响应面法确定的最佳培养基配方为：葡萄糖100.90 g/L、KNO31.57 g/L、乙酸钠3.42 g/L、柠檬酸钠4.56 g/L、酵母膏3.75 g/L、KH2PO42.25 g/L、MgSO4·7H2O 0.60 g/L、CaCl20.30 g/L、FeSO40.15 g/L、ZnSO40.20 g/L。在最佳条件下γ-亚麻酸的产量达1 525.20 mg/L，比初始产量725.90 mg/L提高了1.1倍。 |
| 英文摘要: |
| The nutrients promoting the production of γ-linolenic acid by Mortierella isabellina were found by studying on metabolic regulation of production process of γ-linolenic acid. The medium nutrients were screened by Plackett-Burman experiment.The results showed that the key factors influencing oil yield were glucose，KNO3，sodium acetate and sodium citrate.The four key factors were optimized by response surface methodology with yield of γ-linolenic acid as response value.Combining Plackett-Burman experiment with response surface methodology，the optimal medium formula were obtained as follows：glucose 100.90 g/L，KNO3 1.57 g/L，sodium acetate 3.42 g/L，sodium citrate 4.56 g/L，yeast extract 3.75 g/L，KH2PO42.25 g/L，MgSO4·7H2O 0.60 g/L，CaCl20.30 g/L，FeSO40.15 g/L，ZnSO40.20 g/L.Under the optimal conditions，the yield of γ-linolenic acid reached 1 525.20 mg/L，increasing by 1.1 times than the initial yield (725.90 mg/L) . |
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