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| Optimization of medium for the production of γ-linolenic acid by Mortierella isabellina based on metabolic regulation |
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| DOI： |
| KeyWord:Mortierella isabellina γ-linolenic acid metabolic regulation Plackett-Burman experiment response surface methodology medium optimization |
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| Abstract: |
| The nutrients promoting the production of γ-linolenic acid by Mortierella isabellina were found by studying on metabolic regulation of production process of γ-linolenic acid. The medium nutrients were screened by Plackett-Burman experiment.The results showed that the key factors influencing oil yield were glucose，KNO3，sodium acetate and sodium citrate.The four key factors were optimized by response surface methodology with yield of γ-linolenic acid as response value.Combining Plackett-Burman experiment with response surface methodology，the optimal medium formula were obtained as follows：glucose 100.90 g/L，KNO3 1.57 g/L，sodium acetate 3.42 g/L，sodium citrate 4.56 g/L，yeast extract 3.75 g/L，KH2PO42.25 g/L，MgSO4·7H2O 0.60 g/L，CaCl20.30 g/L，FeSO40.15 g/L，ZnSO40.20 g/L.Under the optimal conditions，the yield of γ-linolenic acid reached 1 525.20 mg/L，increasing by 1.1 times than the initial yield (725.90 mg/L) . |
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