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响应面法优化终极腐霉生产EPA的发酵工艺
Optimization of Pythium ultimum fermentation for EPA production by response surface methodology
  
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中文关键词:  响应面法  终极腐霉  EPA  发酵工艺
英文关键词:response surface methodology  Pythium ultimum  EPA  fermentation process
基金项目:
Author NameAffiliation
LU Meihuan  
LI Lijun  
MA Yinghui，etc  
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中文摘要:
      为进一步提高终极腐霉EPA产量，在初步优化发酵条件的基础上，通过Plackett-Burman和Box-Behnken实验设计优化终极腐霉生产EPA发酵工艺。获得的最优发酵工艺条件为：蔗糖8%，硝酸钾0.4%，酵母粉0.65%，磷酸氢二钠0.175%，硫酸镁0.065%，大豆油1.0%，起始pH 6.0，装液量50 mL/250 mL。在最优发酵工艺条件下，EPA产量达到541.61 mg/L，比初步优化产量（456.39 mg/L）提高了85.22 mg/L。
英文摘要:
      In order to increase the EPA yield produced by Pythium ultimum, the fermentation process of Pythium ultimum for EPA production was optimized by Plackett-Burman design combined with Box-Behnken design on the basis of preliminary optimal fermentation conditions. The results showed that the optimal fermentation conditions were obtained as follows: sucrose 8%, KNO3 0.4%, yeast powder 0.65%, Na2HPO40.175%, MgSO40.065%, soybean oil 1.0%, initial pH 6.0, liquid volume in flask 50 mL/250 mL.Under the optimal conditions, EPA yield reached 541.61 mg/L, which increased by 85.22 mg/L compared with that of preliminary optimization （456.39 mg/L）.
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