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| 水酶法同时提取文冠果油及蛋白质的研究 |
| Simultaneous extraction of Xanthoceras sorbifolia Bunge oil and protein by aqueous enzymatic method |
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| DOI： |
| 中文关键词: 文冠果油 文冠果蛋白 正交实验 脂肪酸组成 氨基酸组成 |
| 英文关键词:Xanthoceras sorbifolia Bunge oil Xanthoceras sorbifolia Bunge protein orthogonal experiment fatty acid composition amino acid composition |
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| 中文摘要: |
| 采用水酶法同时提取文冠果油及蛋白质，以油脂得率及蛋白质得率为指标，采用单因素实验对酶种类、酶解pH、酶解温度、料液比、酶解时间与加酶量6个因素进行研究，并通过正交实验得到最佳工艺。实验得到的最佳酶解条件为：果胶酶与纤维素酶复合酶配比2∶?1，酶解pH 5，酶解温度50?℃，料液比1∶?5，酶解时间4 h，加酶量1 500 U/g。在最佳条件下油脂得率为35.41%，蛋白质得率为14.43%。文冠果油脂肪酸测定结果表明，文冠果油中含棕榈酸4.60%，硬脂酸2.12%，油酸31.28%，亚油酸40.86%，芥酸8.88%。文冠果蛋白氨基酸测定结果表明，文冠果蛋白氨基酸种类齐全，其中谷氨酸含量最高（占氨基酸总量的18.86%），其次为丝氨酸和甘氨酸（分别占氨基酸总量的8.01%、7.16%），必需氨基酸总量达7.91 mg/100 mg，占氨基酸总量的24.10%。 |
| 英文摘要: |
| Xanthoceras sorbifolia Bunge oil and protein were simultaneously extracted by aqueous enzymatic method.With yields of oil and protein as indexes，six factors (types of enzyme，enzymolysis pH，enzymolysis temperature，ratio of solid to liquid，enzymolysis time and enzyme dosage) were studied by single factor experiment，and the extraction conditions were optimized by orthogonal experiment as follows：ratio of pectinase to cellulose 2∶?1，enzymolysis pH 5，enzymolysis temperature 50?℃，ratio of solid to liquid 1∶?5，enzymolysis time 4 h and enzyme dosage 1 500 U/g.Under the optimal conditions，the yields of Xanthoceras sorbifolia Bunge oil and protein reached 35.41% and 14.43%，respectively.Fatty acid composition analysis results indicated that Xanthoceras sorbifolia Bunge oil contained 4.60% of palmitic acid，2.12% of stearic acid，31.28% of oleic acid，40.86% of linoleic acid and 8.88% of erucic acid.Amino acid analysis results showed that Xanthoceras sorbifolia Bunge protein had complete amino acid composition，in which the content of glutamic acid was the highest (18.86% of total amino acids)，followed by serine(8.01% of total amino acids) and glycine (7.16% of total amino acids). The total content of essential amino acids reached 7.91 mg/100 mg，accounting for 24.10% of total amino acids. |
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