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| Preparation of biodiesel from tung oil catalyzed by immobilized lipase |
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| DOI： |
| KeyWord:magnetic Fe3O4 nano-particles immobilized lipase tung oil biodiesel transesterification |
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| Abstract: |
| The preparation of biodiesel from tung oil catalyzed by magnetic Fe3O4 nano-particles containing immobilized lipase was studied, and the effects of dosage of borate buffer，dosage of enzyme，molar ratio of methanol to oil，reaction temperature and cleaning immobilized lipase or not on transesterification were investigated, and the operational stability of the immbilized lipase was studied. The results showed that under the conditions of volume ratio of hexane to tung oil 2∶?1，molar ratio of methanol to oil 1∶?1 at 0，5，12 h (total molar ratio of methanol to oil 3∶?1)，dosage of borate buffer 6%(based on the volume of tung oil)，dosage of enzyme 7.5 mg/mL (based on the volume of tung oil) ，cleaning immobilized lipase by acetone before adding methanol，reaction temperature 45?℃，reaction time 26 h and rotational speed 200 r/min，the conversion rate of methyl ester reached 91.2%.The conversion rate of methyl ester still reached 84.1% after ten batches reactions catalyzed by the immobilized lipase, and it indicated that the immobilized lipase had a certain value of industrial applications. |
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