The immobilized lipase Lipozyme 435 was used as catalyst to catalyze the alcoholysis of high oleic sunflower seed oil(HOSO) and absolute ethanol in a non-solvent system to synthesize 1,2-diacylglyceride(DAG). The optimal reaction conditions were obtained by single factor experiment as follows: molar ratio of HOSO to absolute ethanol 1∶?50, dosage of enzyme 6%(based on the total mass of substrates), reaction temperature 50?℃, reaction time 1 h. Under the optimal conditions, the yield of 1,2-DAG reached 88%.The purity of 1,2-DAG reached 100% after two-step solvent crystallization of crude product. |