|
| Effect of 35.5 kDa subunit lacking on heating behavior of arachin |
| |
| DOI： |
| KeyWord:arachin subunit lacking relative molecular weight distribution thermal stability heat-induced aggregation |
| FundProject: |
|
| Hits: 1238 |
| Download times: 0 |
| Abstract: |
| The relative molecular weight distribution, thermal stability, heat-induced aggregation and radius of gyration of 35.5 kDa subunit lacking arachin were compared with those of normal arachin to study the effect of 35.5 kDa subunit lacking on heating behavior of arachin. The results showed that different molecular species existed in the 35.5 kDa subunit lacking arachin. The lacking of 35.5 kDa subunit could increase thermal stability and denature-cooperativity of arachin. Soluble aggregation formed at 60?℃ in subunit lacking and normal arachin, while covalent aggregation formed at 90?℃and 120?℃. Heat treatment could lead to a looseness of arachin molecular structure and that of subunit lacking arachin was much more loosen than that of normal arachin.The study could provide the basis for screening protein which was suit for heating and the guidance for directed regulation in thermal processing. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
