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| Treatment of cottonseed meal by solid-state fermentation and enzymatic hydrolysis |
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| KeyWord:cottonseed meal solid-state fermentation enzymatic hydrolysis free gossypol nutritional component |
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| Abstract: |
| The changes of toxic factors and nutritional components in cottonseed meal treated by solid-state fermentation and enzymatic hydrolysis were studied. The free gossypol, crude protein, acid soluble protein, crude fiber, amino acid composition and viable count of probiotics were determined in the system of solid-state fermentation and enzymatic hydrolysis participated by Bacillus subtilis GJ00141, Saccharomyces cerevisiae GJ00079 and neutral proteinase. The results showed that compared with the original cottonseed meal,after solid-state fermentation and enzymatic hydrolysis for 60 h, the contents of free gossypol and crude fiber decreased by more than 40% and 12.7% respectively, the acid soluble protein increased by 108.7%, the viable count of Bacillus subtilis and Saccharomyces cerevisiae were 7.75, 7.00 lg(CFU/g) respectively, and the contents of crude protein and total amino acid unchanged. Therefore, the nutritional value and feed quality of cottonseed meal were improved by fermentation and enzymatic hydrolysis. |
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