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| Cloning and functional analysis of omega-3 fatty acid desaturase gene from Paeonia delavayi |
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| DOI： |
| KeyWord:Paeonia delavayi omega-3 fatty acid desaturase gene clone gene functional analysis |
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| Abstract: |
| Omega-3 fatty acid desaturase is the key enzyme in the biosynthesis pathway of linolenic belonging to polyunsaturated fatty acids. A full-length cDNA sequences of a new FAD3 gene was successfully cloned from Paeonia delavayi by RT-PCR method with the specific primers designed based on transcriptome datas, named PdFAD3(GeneBank accession number: KX289610).Bioinformatics analysis results showed that full-length sequence of PdFAD3 gene was 2 134 bp, containing the complete cDNA open reading frame (ORF), encoded a protein with 450 amino acids. Blast comparison results showed that the protein belonged to the FAD family. Analysis results of phylogenetic tree showed that Paeonia delavayi had close genetic relationship with Paeonia lactiflora belonging to Paeonia Sect. Paeonia. The protein belonged to the transmembrane protein and was less hydrophilic. RT-PCR results showed that the expression in the seed as maturation occured bimodal change, similar to the FAD3 reported in other species. The PdFAD3 gene cloned from Paeonia delavayi provided a scientific basis for further study on its regulation mechanism and obtaining Paeonia delavayi transgenic variety with highly unsaturated fatty acids. |
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