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| Effect of the secondary culture on contents of total lipids，EPA and PUFA of Tropidoneis maxima |
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| KeyWord:Tropidoneis maxima secondary culture total lipids EPA PUFA |
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| Abstract: |
| To explore the effects of the secondary culture (low temperature and high light intensity stress)on the contents of total lipids， EPA and PUFA of Tropidoneis maxima， Tropidoneis maxima was cultured for 10 d under the conditions of temperature 25?℃， light intensity 36 μmol/(m2·s) and salinity 25， then was secondarily cultured under low temperature(10?℃) or high light intensity (80 μmol/(m2·s)) by single factor experiment， and the two-factor three-level secondary culture experiment of low temperature and high light intensity was further conducted.The results showed that the optimal secondary culture time was 3 d；low temperature of 10?℃ was beneficial to the accumulation of EPA and PUFA， and they could reach (28.48±0.47)% and (43.28±1.06)%， respectively；the light intensity of 80 μmol/(m2·s) also had a positive effect on the accumulation of total lipids， EPA and PUFA， reaching(43.19±1.29)%， (27.11±0.25)% and (41.78±0.22)%， respectively. And it was more beneficial to the accumulation of total lipids， EPA and PUFA under the interaction between low temperature 10?℃ and light intensity 80 μmol/(m2·s)， and their contents were (42.99±2.51)%， (32.83±0.58)% and (46.43±0.22)%， respectively. Above all， the secondary culture was favorable to obtain higher levels of growth and lipid accumulation of algae. It provided a novel culture method for improving lipid production of algae， and was worthy for further study. |
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