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Deacidification of adlay bran oil with high acid value and its activity |
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KeyWord:adlay bran oil alkali refining deacidification response surface methodology activity |
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Abstract: |
In order to improve the quality and reduce the acid value of adlay bran oil, the alkali refining deacidification conditions of adlay bran oil were optimized by response surface methodology, and the activity of refined adlay bran oil was studied. The results showed that the optimal alkali refining deacidification conditions of adlay bran oil were obtained as follows: excessive alkali 0.21%, alkali refining time 33 min, mass concentration of alkali solution 26.58%, and alkali refining temperature 58?℃. Under these conditions, the acid value of adlay bran oil reduced from 45.9 mgKOH/g to 0.59 mgKOH/g. Besides, the refined adlay bran oil had dosage-dependant relationship with inhibitory rate of tyrosinase in the mass concentration of 0.75-12 mg/mL, and the IC50 was 2 mg/mL. The IC50 of refined adlay bran oil against DPPH radical was 6.36 mg/mL. It turned out that adlay bran oil had certain whitening effect and antioxidant activity. In a word, refined adlay bran oil was a kind of natural vegetable oil with high quality and biological activity, which might be developed as functional oil in the future. |
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