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Enzymatic preparation of arachidonoyl ethanolamide
  
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KeyWord:arachidonic acid  two step enrichment  arachidonoyl ethanolamide  enzymatic preparation
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WANG Yingying, WANG Xiaosan, JIN Qingzhe, WANG Xingguo  
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Abstract:
      With arachidonic acid(ARA)as acyl donor, the enzymatic preparation of arachidonoyl ethanolamide(AEA)was studied. ARA was enriched by two step enrichment using urea adduct method and solvent extraction method. The optimal reaction conditions of enzymatic preparation of AEA were obtained as follows: 1 mmol mixed fatty acid(ARA content 84.68%)reacted with 1 mmol ethanolamine in 1.5 mL hexane under the catalysis of 6% Lipozyme 435(based on total mass of reaction substrate), 50?℃ and 400 r/min for 6 h. Under these conditions, the purity of AEA was 82.69%.
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