Subcritical extraction of ginkgo seed oil
  
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KeyWord:ginkgo seed oil  subcritical extraction  physicochemical index  fatty acid composition
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ZHOU Hao, WANG Chengzhang, YE Jianzhong, CHEN Hongxia, TAO Ran, DAI Mingming  
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Abstract:
      With ginkgo seed as raw material, subcritical butane as extraction solvent and oil yield of ginkgo seed as evaluation index, the subcritical extraction technology of ginkgo seed oil was optimized by single factor experiment and orthogonal experiment. The results showed that the optimal subcritical extraction conditions of ginkgo seed oil were obtained as follows: extraction pressure 0.5 MPa, extraction temperature 40?℃, extraction time 60 min, ratio of material to liquid 1∶?6 and extraction times three. Under these conditions, the oil yield of ginkgo seed was 96.16%.The detection results of physicochemical properties of ginkgo seed oil indicated that the relative density, refractive index(20?℃), acid value, iodine value, peroxide value, and saponication value were 0.922 5, 1.473 7, 1.95 mgKOH/g, 156.62 gI/100 g, 1.46 mmol/kg and 181.19 mgKOH/g, respectively. The fatty acid composition of ginkgo seed oil was analyzed by GC-MS and six kinds of fatty acids were identified as oleic acid (30.25%), linoleic acid (50.04%), palmitic acid (8.28%), 5, 12-octadecadienoic acid(2.34%), 9-hexadecenoic acid(4.41%)and 5, 11, 14-eicosatrienoic acid (4.67%), in which the content of unsaturated fatty acids was 91.71%.
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