Optimization of ultrasound-assisted extraction of flavonoids from seabuckthorn seed meal by response surface methodology
  
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KeyWord:seabuckthorn flavonoids  ultrasound-assisted extraction  response surface methodology
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ZHANG Hairong, SHI Zhenhua  
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Abstract:
      The ultrasound-assisted extraction of flavonoids from seabuckthorn seed meal was investigated. Based on single factor experiment, the factors such as ultrasonic power, extraction time, ratio of liquid to material, extraction temperature and volume fraction of ethanol, influencing yield of flavonoids were optimized by response surface methodology, and predictive polynomial quadratic equation model was established. The results showed that the optimal extraction conditions were obtained as follows:ultrasonic power 60 W, extraction time 20 min, ratio of liquid to material 30∶?1, extraction temperature 54?℃, volume fraction of ethanol 59%, extraction times twice. Under these conditions, the yield of flavonoids reached 7.11%, and it was consistent with model theoretical value of 7.08%.
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