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| 醇洗法提取杜仲籽仁粕中桃叶珊瑚苷及制取浓缩蛋白 |
| Preparation of aucubin and protein concentrate from eucommia seed kernel meal by alcohol leaching method |
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| DOI： |
| 中文关键词: 杜仲籽 杜仲籽仁粕 桃叶珊瑚苷 醇洗 浓缩蛋白 |
| 英文关键词:eucommia seed kernel eucommia seed kernel meal aucubin alcohol leaching protein concentrate |
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| 中文摘要: |
| 以杜仲籽仁粕为原料，利用醇洗工艺提取桃叶珊瑚苷同时制备浓缩蛋白，并采用高效液相色谱法（HPLC-UV）检测杜仲籽仁及其粕、浓缩蛋白中桃叶珊瑚苷含量。结果表明：不同产地杜仲籽仁及其粕中桃叶珊瑚苷含量分别为6.86%～15.44%、5.25%～16.60%。利用含水乙醇对杜仲籽仁粕进行醇洗时，浓缩蛋白和提取液均会变黑，且基本检测不出桃叶珊瑚苷。只有用无水乙醇作为醇洗溶剂时，才能得到乳白色的浓缩蛋白和富含桃叶珊瑚苷的提取液，此时浓缩蛋白的蛋白质含量达到51.78%，较杜仲籽仁粕中提高16.54个百分点。以无水乙醇为溶剂提取杜仲籽仁粕中的桃叶珊瑚苷，最佳提取条件为提取温度80?℃、提取时间20 min、液料比8∶?1、提取次数2次。在最佳提取条件下，桃叶珊瑚苷的提取率达到94.82%。 |
| 英文摘要: |
| With eucommia seed kernel meal as raw material, aucubin and protein concentrate were prepared from eucommia seed kernel meal by alcohol leaching method. The aucubin contents in eucommia seed kernel, meal and protein concentrate were determined by HPLC-UV. The results showed that the aucubin contents in emucommia seed kernel and its meal from different areas were 6.86%-15.44% and 5.25%-16.60% respectively. The protein concentrate and the extract were black and the aucubin could not be detected when the extraction solvent was hydrous ethanol. Only with anhydrous ethanol as extraction solvent, the milky white protein concentrate could be obtained and the extract was rich in aucubin. And the content of protein in protein concentrate was 51.78%, which increased by 16.54 percentage points than eucommia seed kernel meal. The aucubin in the meal was extracted by anhydrous ethanol, and the optimal extraction conditions were obtained as follows: extraction temperature 80?℃, extraction time 20 min, ratio of liquid to material 8∶?1, extraction times twice. Under these conditions, the extraction rate of aucubin reached 94.82%. |
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