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| Production of microbial oil and bacterial protein from beer production wastewater fermented by yeast |
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| DOI： |
| KeyWord:beer production wastewater oleaginous yeast microbial oil bacterial protein COD fatty acid composition essential amino acid |
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| Abstract: |
| For exploring the comprehensive utilization method and way of beer production wastewater, using the liquid fermentation to select high-yield strains which could product microbial oil, bacterial protein and could effectively degrade COD from seven yeast strains were studied. The fermentation period was examined and the fatty acid composition of microbial oil and amino acid composition of bacterial protein of the screened yeast strains were analyzed. The results showed that the resource utilization degrees of 6# strain (Trichosporon porosum D02) and 1# strain (Lipomyces starkeyi 1809) on wastewater were the highest. When two screened strains 6# strain and 1# strain were fermented for 120 h, the oil productivity, crude bacterial protein productivity and degradation rate of COD were 8.93 g/L, 6.1 g/L, 89.09% and 4.62 g/L, 4.42 g/L, 81.47% respectively. C18 and C16 fatty acids were the main components in the fatty acid composition of microbial oil of two screened strains. The contents of oleic acid and palmitic acid were the highest. The indexes of unsaturated fatty acid (IUFA) were 80.33% and 61.57% respectively. Except proline was not detected, the bacterial protein of two screened strains contained 17 kinds of amino acids and the contents of eight kinds of essential amino acids were 44.14% and 44.23% respectively. Bacterial protein could be used as a new source of animal protein feed. |
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