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Microwave-assisted extraction and antioxidant activity in vitro of oil-tea camellia seed polysaccharides |
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KeyWord:oil-tea camellia seed polysaccharides microwave-assisted extraction antioxidant activity |
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Abstract: |
The microwave-assisted technology was applied to extract polysaccharides from oil-tea camellia seed meal.According to single factor experiment, the extraction conditions of oil-tea camellia seed polysaccharides were optimized by orthogonal experiment. And the in vitro antioxidant activity of oil-tea camellia seed polysaccharides was studied. The results showed that the optimal extraction conditions of oil-tea camellia seed polysaccharides were obtained as follows:microwave power 800 W, microwave time 6.5 min, ratio of material to liquid 1∶?60, extraction time 2 h, extraction temperature 100?℃. Under these conditions, the yield of oil-tea camellia seed polysaccharides was (7.61±0.5)%. The oil-tea camellia seed polysaccharides had certain scavenging activity on hydroxyl free radical, DPPH free radical and nitrites, nevertheless, its scavenging ability on superoxide anion free radical and reducing ability were weak. |
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