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| Optimization of Lipozyme 435-catalyzed synthesis of medium-and long-chain triglycerides using response surface methodology |
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| KeyWord:medium-and long-chain triglycerides Lipozyme 435 interesterification response surface methodology triglycerides structure |
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| Abstract: |
| The synthesis conditions of medium- and long-chain triglycerides by Lipozyme 435-catalyzed interesterification of soybean oil and medium-chain triglycerides in a solvent-free system was optimized by response surface methodology. The triglycerides structure of the product was analyzed by ultra-performance liquid chromatography (UPLC) coupled with quadrupole time of flight mass spectrometry under positive ion model. The results showed that reaction temperature and reaction time had extremely significant influence on medium-and long-chain triglycerides content. The optimal reaction conditions were obtained as follows： mass ratio of soybean oil to medium-chain triglycerides 60∶?40, reaction time 4.36 h, reaction temperature 89?℃, and enzyme dosage 5.5%(based on the mass of substrate). Under these conditions, medium- and long-chain triglycerides content was 84.15%. The main triglycerides in the products were CyCyL, CyCyLn, LOCy, LPCy, OOCy and LOCa. |
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