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| Effects of different culture conditions on the growth and accumulations of lipid and arachidonic acid of Loboshpaera incisa |
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| KeyWord:Lobosphaera incisa biomass lipid arachidonic acid |
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| Abstract: |
| In order to increase the yield of Lobosphaera incisa, the effects of light intensity, initial nitrogen concentration and medium replacement on the growth and accumulations of lipid and arachidonic acid were investigated. The results showed that under the same light intensity, L. incisa got higher biomass under the condition of high nitrogen concentration, while the contents of total lipid and arachidonic acid were lower. Replacing the medium under the bilateral high light condition, the highest biomass reached 7.36 g/L with the medium raplacement from high nitrogen concentration to high nitrogen concentration. In contrast, the highest contents of total lipid and arachidonic acid were 47.74% and 6.18% respectively which got through replacing the low nitrogen concentration medium with the nitrogen free medium. In conclusion, the cultivation partten of changing from high nitrogen concentration to high nitrogen concentration was the most benificial to the growth of L.incisa, but the productivity of arachidonic acid was only 7.0 mg/(L路d). However, other cultivation parttens (replacing high nitrogen concentration with nitrogen free, replacing low nitrogen concentration with low nitrogen concentration, replacing low nitrogen concentration with nitrogen free) promoted the accumulation of arachidonic acid, and the productivities were 14.0, 15.3, 17.0 mg/(L路d), respectivety. The research indicated that L. incisa was a potential strain for the production of arachidonic acid . |
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