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| 酸解巴沙鲶鱼油制备富含OPO的人乳替代脂 |
| Production of structured lipids rich in 1,3-dioleoyl-2-palmitoylglycerol from basa catfish oil as human milk fat substitutes by enzymatic acidolysis |
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| DOI： |
| 中文关键词: 1,3-二油酸-2-棕榈酸甘油三酯（OPO） 巴沙鲶鱼油 酸解 Lipozyme RM IM |
| 英文关键词:1, 3-dioleoyl-2-palmitoylglycerol（OPO） basa catfish oil acidolysis Lipozyme RM IM |
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| 中文摘要: |
| 以巴沙鲶鱼油为原料、高油酸葵花籽油来源的游离脂肪酸为酰基供体，采用Lipozyme RM IM脂肪酶为催化剂催化酸解反应制备富含OPO的人乳替代脂。并采用液质联用对巴沙鲶鱼油、酸解巴沙鲶鱼油、市售BetapolTM的sn-OPO含量进行分析。结果表明，最佳制备条件为：底物摩尔比1∶?6，酶用量12%，反应温度60?℃，水分含量3.5%（基于酶质量），反应时间3 h。在最佳条件下，酸解产物中sn-2棕榈酸分布和sn-1，3油酸含量分别为73.18%和71.11%。酸解产物中sn-OPO含量从23.35%提高到34.28%，高于市售BetapolTM的33.24%。 |
| 英文摘要: |
| Lipozyme RM IM-catalyzed acidolysis of basa catfish oil with free fatty acids from high oleic acid sunflower seed oil to increase the content of OPO was conducted. The sn-OPO contents of basa catfish oil, enzymatic product and BetapolTM were determined by UPLC-MS. The results showed that the optimal preparation conditions were obtained as follows: molar ratio of substrate 1∶?6, enzyme dosage 12%, reaction temperature 60?℃, water content 3.5%(on the basis of enzyme mass) and reaction time 3 h. Under these conditions, the distribution of sn-2 palmitic acid and the content of sn-1，3 oleic acid in the acidolysis product were 73.18% and 71.11%, respectively. The content of sn-OPO in the acidolysis product increased from 23.35% to 34.28%, higher than 33.24% in BetapolTM. |
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