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Comparison of determination of fat content in margarine by acid hydrolysis and automatic Soxhlet extraction and method improvement |
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KeyWord:margarine acid hydrolysis automatic Soxhlet extration improvement t test |
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Abstract: |
Fat contents in six kinds of margarine were determined by acid hydrolysis and automatic Soxhlet extraction, and the automatic Soxhlet extraction was improved. The results of different methods were comparatively analyzed and researched. The results showed that the experimental result of acid hydrolysis was lower. There was a significant difference between acid hydrolysis and automatic Soxhlet extraction in the determination of fat content in margarine. So the automatic Soxhlet extraction was more appropriate. In the same time, the automatic Soxhlet extraction was improved, and the experimental result had high accuracy with the standard deviation 0.12 and the standard error of mean 0.03, showing that the reproducibility was good. The improvement method had a significant impact on the experiment. Moreover, the extraction paper tube could be recycled for twelve times, which reduced the cost of experiment by the improvement method. |
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