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Determination method of oryzanol in rice bran oil
  
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KeyWord:rice bran oil  oryzanol  potassium hydroxide  ethanol  colorimetric method
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LI Runfeng，CHANG Xuedong，ZHANG Yaping，GUO Lingling  
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Abstract:
      The determination conditions of colorimetric method of potassium hydroxide in ethanol were studied with oryzanol in rice bran oil as subject. The precision, recovery and stability were measured to evaluate the method. The results showed that using colorimetric method of potassium hydroxide in ethanol for determining oryzanol in rice bran oil, the best solvent was chloroform or petroleum ether. When the mass concentration of oryzanol was in the range of 4-20 關g/mL, the linear correlation between mass concentration and absorbance was good, and the correlation coefficient(R) was 0.999 4. The recoveries of standard addition of oryzanol in soybean oil and rice bran oil and relative standard deviation (RSD) were above 99% and below 0.5%, respectively. The method also had high precision and good repeatability.
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