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| 水酶法提取胡麻籽油的工艺研究 |
| Aqueous enzymatic extraction process of flaxseed oil |
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| DOI： |
| 中文关键词: 水酶法 复合酶 响应面法；胡麻籽油 |
| 英文关键词:aqueous enzymatic method compound enzyme response surface methodology flaxseed oil |
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| 中文摘要: |
| 以胡麻籽为原料，采用复合酶酶解技术对胡麻籽油进行提取，考察了预处理方法、粉碎时间、酶的种类、复合酶复配比例及复合酶添加量对胡麻籽油提取率的影响，在前期实验的基础上，采用中心组合设计，对液料比、酶解pH、酶解温度及酶解时间进行了响应面优化。结果表明：高压高温湿热法预处理，胡麻籽在10 000 r/min转速下粉碎90 s利于胡麻籽油的提取；最佳复合酶制剂组成为中性蛋白酶与戊聚糖酶质量比2∶?1，复合酶的最适添加量为1.2%（以脱壳胡麻籽质量计）；响应面优化的最适工艺参数为液料比9.19∶?1、酶解pH 5.94、酶解温度53.62?℃、酶解时间3.20 h；在最适工艺条件下，胡麻籽油提取率可达86.73%。 |
| 英文摘要: |
| With flaxseed as raw material, flaxseed oil was extracted by compound enzymatic hydrolysis technology. The effects of pretreatment methods, grinding time, enzyme types, combination proportion of compound enzyme and dosage of compound enzyme on the extraction rate of flaxseed oil were studied. Based on the previous experiments, the ratio of liquid to material, enzymatic hydrolysis pH, enzymatic hydrolysis temperature and enzymatic hydrolysis time were optimized by response surface method. The results showed that high pressure and high temperature hydro-thermal pretreatment and grinding for 90 s at 10 000 r/min were beneficial for extraction of flaxseed oil. The optimal compound enzyme composition was mass ratio of neutrase to pentosanase 2∶?1 and the optimal dosage was 1.2% (based on the mass of dehulled flaxseed). The optimal extraction parameters of flaxseed oil were obtained as follows: ratio of liquid to material 9.19∶?1, enzymatic hydrolysis pH 5.94, enzymatic hydrolysis temperature 53.62?℃ and enzymatic hydrolysis time 3.20 h. Under these conditions, the extraction rate of flaxseed oil could reach 86.73%. |
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