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| Aqueous enzymatic extraction process of flaxseed oil |
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| DOI： |
| KeyWord:aqueous enzymatic method compound enzyme response surface methodology flaxseed oil |
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| Abstract: |
| With flaxseed as raw material, flaxseed oil was extracted by compound enzymatic hydrolysis technology. The effects of pretreatment methods, grinding time, enzyme types, combination proportion of compound enzyme and dosage of compound enzyme on the extraction rate of flaxseed oil were studied. Based on the previous experiments, the ratio of liquid to material, enzymatic hydrolysis pH, enzymatic hydrolysis temperature and enzymatic hydrolysis time were optimized by response surface method. The results showed that high pressure and high temperature hydro-thermal pretreatment and grinding for 90 s at 10 000 r/min were beneficial for extraction of flaxseed oil. The optimal compound enzyme composition was mass ratio of neutrase to pentosanase 2∶?1 and the optimal dosage was 1.2% (based on the mass of dehulled flaxseed). The optimal extraction parameters of flaxseed oil were obtained as follows: ratio of liquid to material 9.19∶?1, enzymatic hydrolysis pH 5.94, enzymatic hydrolysis temperature 53.62?℃ and enzymatic hydrolysis time 3.20 h. Under these conditions, the extraction rate of flaxseed oil could reach 86.73%. |
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