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| 植物甾醇酯对高脂膳食大鼠脂代谢影响及其机制研究 |
| Effect of phytosterol ester on lipid metabolism of rats fed a high fat diet and its mechanism |
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| DOI： |
| 中文关键词: 植物甾醇酯 高脂膳食大鼠 降脂 CYP7A1 PPARγ SIRT1 |
| 英文关键词:phytosterol ester high fat diet rat lipid-lowering CYP7A1 PPAR款 SIRT1 |
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| 中文摘要: |
| 为了研究植物甾醇酯(PSE)对高脂膳食大鼠肝脏脂代谢的作用及其机制，将42只雌性SD大鼠随机均分为对照组、模型组和干预组，分别给予基础饲料、高脂饲料和高脂加质量分数2% PSE饲料24周。监测大鼠的体重；检测血清脂蛋白的水平和肝脏脂质水平；检测血清转氨酶（ALT、AST）活性并对肝脏结构进行病理学观察；分别用Real-time PCR和Western Blot的方法评估肝脏LXRα、CYP7A1、ABCA1、HMGCoA、FXR、SREBP-1c、 PPARγ、SIRT1 mRNA的表达水平以及PPARγ和SIRT1蛋白的表达水平。结果表明，PSE降低了血清TC和LDL-C以及肝脏TC的水平，增加了血清HDL-C的水平(P＜0.05)，改善了由高脂膳食导致的肝脏组织病理学改变，降低了血清ALT和AST的水平(P＜0.05)。PSE增加了肝脏SIRT1 mRNA的表达，减少了CYP7A1 mRNA和PPARγ mRNA以及PPARγ蛋白的表达(P＜0.05)，对LXRα、ABCA1、HMGCoA、FXR和SREBP-1c mRNA的表达以及SIRT1蛋白的表达没有显著作用。 |
| 英文摘要: |
| The effect of phytosterol ester(PSE) on lipid metabolism of rats fed a high fat diet and its mechanism were investigated. 42 female SD rats were randomly divided into a control group, a model group and an intervening group, fed with basic feed, high fat diet (HFD) and HFD with 2% PSE respectively for 24 weeks. The changes of weight were monitored, the levels of lipoprotein in serum and liver were measured, the activities of ALT and AST in serum were determined and the pathological changes of the liver were observed. The mRNA expressions of LXRα, CYP7A1, ABCA1, HMGCoA, FXR, SREBP-1c, PPARγ and SIRT1 were measured by Real-time PCR and Western Blot. The results showed that PSE significantly lowered serum TC, LDL-C and liver TC level，increased serum HDL-C level (P＜0.05), attenuated HFD-induced histopathological changes of liver and reduced the serum ALT and AST activity (P＜0.05). PSE had no effect on the mRNA level of LXRα, ABCA1, HMGCoA, FXR and SREBP-1c and protein level of SIRT1, but PSE increased SIRT1 mRNA in liver and decreased CYP7A1 mRNA. Most importantly, PSE decreased not only the protein expression of PPARγ but also expression of PPARγ mRNA. |
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