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| Optimization of salt extraction of protein from Zanthoxylum bungeanum Maxim. seed kernel by response surface methodology |
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| KeyWord:Zanthoxylum bungeanum Maxim.seed kernel protein salt extraction response surface methodology |
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| Abstract: |
| The salt extraction of protein from Zanthoxylum bungeanum Maxim.seed kernel was investigated, and the effects of extraction time, solid-liquid ratio, NaCl concentration, extraction temperature and extraction pH on the extraction rate of protein were discussed. On the basis of single factor experiment, the extraction conditions of protein from Zanthoxylum bungeanum Maxim.seed kernel were optimized by response surface methodology. The results showed that fixing extraction pH at 11, extraction time had the largest effect on the extraction rate of protein from Zanthoxylum bungeanum Maxim.seed kernel, followed by extraction temperature, solid-liquid ratio and NaCl concentration. The optimal extraction conditions were determined as follows: extraction pH 11, extraction time 35 min, extraction temperature 50?℃, solid-liquid ratio 1∶?21, and NaCl concentration 1.2 mol/L. Under these conditions, the extraction rate of protein from Zanthoxylum bungeanum Maxim.seed kernel was 88.77%, and the content of the protein reached 93.17%. |
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