| In order to extract Hibiseu smanihot L. seed oil (HSO), response surface methodology was used to optimize the conditions of supercritical carbon dioxide extraction of Hibiseu smanihot L. seed oil. Based on single factor experiment, with extraction pressure, extraction temperature and extraction time as factors and yield of Hibiseu smanihot L. seed oil as index, the mathematical model was established by central composite experiment (Box-Behnken) for response surface analysis, and the fatty acid composition of Hibiseu smanihot L. seed oil was analyzed. The results showed that the optimal extraction condition were obtained as follows: extraction pressure 32 MPa, extraction temperature 40?℃, and extraction time 120 min. Under the optimal conditions, the yield of Hibiseu smanihot L. seed oil was （22.9±0.2）%. The main fatty acids of Hibiseu smanihot L. seed oil were unsaturated fatty acids, which accounted for 76.12%, and the contents of palmitoleic acid, linoleic acid and oleic acid were 0.58%, 35.65% and 39.89% respectively. The research indicated that supercritical carbon dioxide extraction was a suitable method for extracting Hibiseu smanihot L. seed oil and Hibiseu smanihot L. seed oil could be developed as edible healthy oil. |
