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| Preparation of procyanidins of different purities from peanut skins |
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| KeyWord:peanut skin procyanidins macroporous adsorption resin purity oligomer |
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| Abstract: |
| The preparation process of procyanidins of different purities from peanut skins with AB-8 macroporous adsorption resin was studied and the product was analyzed by HPLC-MS. The results showed that the optimal separation and purification conditions were obtained as follows: flow rate of sample 0.5 mL/min, eluention flow rate 1.0 mL/min, 20% and 40% ethanol solution as eluent. Under these conditions, the procyanidins purities after desorption were 98.7% and 86.2% respectively. In addition, procyanidins of different purities were obtained by different collection methods. Purified products separated by AB-8 macroporous adsorption resin were oligomeric procyanidins. The main purified products of 20% ethanol were A-type dimers of procyanidins and A-type trimers of procyanidins, and the main purified products of 40% ethanol were A-type dimers of procyanidins and A-type tetramers of procyanidins. |
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