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Preparation of walnut oil by ultrasound-assisted complex enzymes method
  
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KeyWord:ultrasound  complex enzymes  walnut oil
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HE Qiushi，WANG Zhixing，ZHAO Chengbin  
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Abstract:
      Using walnut kernel as raw material, wall-breaking treatment was carried out by ultrasound-assisted complex enzymes (pectase, cellulase, hemicellulase) method, and walnut oil was prepared by enzymolysis of alkaline protease. The process conditions of ultrasound-assisted enzymolysis and the ratio of complex enzymes were studied. The results showed that the oil extraction effect of complex enzymes was better than that of single enzyme. The optimal enzymolysis wall-breaking conditions were obtained as follows: ultrasonic power 90 W, dosage of composite enzymes 4%, enzymolysis pH 4.5, enzymolysis temperature 50?℃ and enzymolysis time 40 min. Under these conditions, the ratio of complex enzymes was optimized by mixture experiment and the optimal ratio of complex enzymes(pectase, cellulase, hemicellulase) was determined as 1∶1.46∶1.11. Under these conditions, the total extraction rate of oil was the highest, which was 89.73%.
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