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超声辅助复合酶法制备核桃油工艺研究
Preparation of walnut oil by ultrasound-assisted complex enzymes method
  
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中文关键词:  超声波  复合酶  核桃油
英文关键词:ultrasound  complex enzymes  walnut oil
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Author NameAffiliation
HE Qiushi，WANG Zhixing，ZHAO Chengbin  
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中文摘要:
      以核桃仁为原料，采用超声辅助复合酶（果胶酶、纤维素酶、半纤维素酶）破壁，然后进行碱性蛋白酶酶解制备核桃油，对超声辅助酶解工艺条件及复合酶配比进行研究。结果表明：复合酶破壁的提油效果优于单一酶；最佳酶解破壁条件为超声功率90 W、复合酶添加量4%、酶解pH 4.5、酶解温度50?℃、酶解时间40 min；在最佳的复合酶破壁条件下，利用混料试验对复合酶配比进行优化，确定最佳复合酶（果胶酶、纤维素酶、半纤维素酶）配比为1∶1.46∶1.11，此时总油提取率最大，为89.73%。
英文摘要:
      Using walnut kernel as raw material, wall-breaking treatment was carried out by ultrasound-assisted complex enzymes (pectase, cellulase, hemicellulase) method, and walnut oil was prepared by enzymolysis of alkaline protease. The process conditions of ultrasound-assisted enzymolysis and the ratio of complex enzymes were studied. The results showed that the oil extraction effect of complex enzymes was better than that of single enzyme. The optimal enzymolysis wall-breaking conditions were obtained as follows: ultrasonic power 90 W, dosage of composite enzymes 4%, enzymolysis pH 4.5, enzymolysis temperature 50?℃ and enzymolysis time 40 min. Under these conditions, the ratio of complex enzymes was optimized by mixture experiment and the optimal ratio of complex enzymes(pectase, cellulase, hemicellulase) was determined as 1∶1.46∶1.11. Under these conditions, the total extraction rate of oil was the highest, which was 89.73%.
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