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| 秋刀鱼内脏磷脂的化学特性研究 |
| Chemical characterization of Pacific saury (Cololabis Saira) viscera lecithin |
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| DOI： |
| 中文关键词: 秋刀鱼内脏 磷脂 超临界二氧化碳 脂肪酸 |
| 英文关键词:Pacific saury viscera lecithin SC-CO2 fatty acid |
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| 中文摘要: |
| 采用超临界二氧化碳（SC-CO2）与有机溶剂提取两种方法对秋刀鱼内脏中的磷脂进行提取，研究了两种方法提取的磷脂及SC-CO2提取所得磷脂中的磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）的脂肪酸组成及含量，并对SC-CO2提取磷脂的酸值、过氧化值等理化指标进行检测分析。结果表明：SC-CO2法的提取率(23.90±1.10)%（干重）高于有机溶剂法的提取率(20.81±1.25)%（干重），但两者无显著差异（P＞0.05）；SC-CO2法所得磷脂的脂肪酸总量高于有机溶剂法的，其中二十碳五烯酸（EPA）的含量分别为8.80%和8.95%，二十二碳六烯酸（DHA）的含量分别为43.20%和43.82%；PC、PE的脂肪酸组成之间无显著差异（P＞0.05）；磷脂酸值（KOH）为（21.52±0.57）mg/g，过氧化值为（2.06±0.12）mmol/kg，均符合JECFA标准Lecithin (Phosphalipides, Phospholipids; INS No.322 (ⅰ))的规定。 |
| 英文摘要: |
| The lecithin in Pacific saury (Cololabis Saira) viscera was extracted by supercritical carbon dioxide (SC-CO2) and organic solvent method. Fatty acid compositions and contents of lecithin extracted by the two methods and its phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in lecithin extracted by SC-CO2 were determined. Meanwhile, the acid value and peroxide value of Pacific saury viscera lecithin extracted by SC-CO2 were detected. The results showed that the extraction rate of Pacific saury viscera lecithin of SC-CO22 ((23.90±1.10)%, dry basis) was higher than that of organic solvent method ((20.81±1.25)%, dry basis), but they had no significant difference（P＞0.05）. The total fatty acid content of lecithin obtained by SC-CO22 was higher than that of lecithin obtained by organic solvent method. EPA contents in lecithin obtained by SC-CO2 and organic solvent method were 8.80% and 8.95%, respectively. DHA contents in lecithin obtained by SC-CO2 and organic solvent method were 43.20% and 43.82%, respectively. The fatty acid compositions of PC and PE had no significant difference（P＞0.05）. The acid value and peroxide value of Pacific saury viscera lecithin were (21.52±0.57)mgKOH/g and (2.06±0.12)mmol/kg, respectively, which met the standard of JECFA Lecithin (Phos phalipides, Phospholipids; INS No.322 (ⅰ)). |
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