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| 甘油二酯异构体高效液相色谱分析法的建立 |
| Determination of diacylglycerol positional isomers by high performance liquid chromatography |
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| DOI： |
| 中文关键词: 甘油二酯 位置异构体 高效液相色谱-蒸发光散射检测器 优化 |
| 英文关键词:diacylglycerol positional isomers high performance liquid chromatography- evaporative light scattering detector optimization |
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| 中文摘要: |
| 建立了1-月桂酸-3-棕榈酸甘油二酯和1-月桂酸-2-棕榈酸甘油二酯两种位置异构体的高效液相色谱分析方法。最佳色谱分析条件为蒸发光散射检测器（漂移管温度90℃，N2流速2.2 L/min），流动相为乙腈-异丙醇(体积比95∶5)，流速1.0 mL/min，柱温35℃；最佳色谱条件下，测得 0.05～5.00 mg/mL质量浓度范围内，1-月桂酸-3-棕榈酸甘油二酯的标准曲线方程为Y=1.832X+4.564，线性回归系数R2=0.998 8，线性关系良好；该方法具有良好的精密度、准确度与重现性，适合结构脂质等样品中甘油二酯位置异构体定性、定量分析的要求。 |
| 英文摘要: |
| A HPLC method was developed for the separation of 1-laurin-3-palmitin diacylglycerol and 1-laurin-2-palmitin diacylglycerol. The optimal chromatographic analysis conditions were obtained as follows: evaporative light scattering detection (ELSD) as detector (drift tube temperature 90℃ and N2 flow rate 2.2 L/min), mobile phase of acetonitrile-isopropanol (volume ratio 95∶5), flow rate 1.0 mL/min, column temperature 35℃. Under the optimal chromatographic analysis conditions, the standard curve equation of 1-laurin-3-palmitin diacylglycerol (mass concentration range 0.05-5.00mg/mL) was Y = 1.832X + 4.564 with a good linear regression coefficient (R2=0.998 8). The method showed good reproducibility, precision and accuracy, which was beneficial for the qualitative and quantitative analysis of diacylglycerol positional isomers in structural lipids. |
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