Optimization of oil-tea camellia seed oil nanoemulsion prepared using ultrasonic emulsification by response surface methodology and its stability
  
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KeyWord:ultrasonic emulsification  oil-tea camellia seed oil  nanoemulsion  response surface methodology  stability
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HU Wei, LI Xiangzhou, MU Yuanyuan  
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Abstract:
      With Tween80 as emulsifier, the effects of ultrasonic power, oil- tea camellia seed oil (CSO) volume fraction and ratio of emulsifier to oil (SOR) on the mean droplets diameter (Z-Ave) and polydispersity index (PDI) of the CSO nanoemulsion were investigated. The preparation conditions of CSO nanoemulsion using ultrasonic emulsification were optimized by response surface methodology and its stability was determined. The results showed that R2 of Z-Ave and PDI were 0.974 2 and 0.951 9 respectively. The optimal preparation conditions were obtained as follows: ultrasonic power 405 W, ultrasonic time 15 min, CSO volume fraction 8.3% and SOR 0.16∶?1. Under these conditions, Z-Ave was (74.9±0.85)nm and PDI was 0.17±0.01. Moreover, the effects of storage time and storage temperature as well as their interactions on the Z-Ave and PDI were highly significant (P<0.01). CSO nanoemulsion stored at 5℃ and 25℃ for 60 days, Z-Ave was less than 90 nm, and PDI was less than 0.3. It showed good dynamic stability.
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