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Ultrasound-assisted extraction of Benincasa hispida Cogn. seed oil and its fatty acid composition analysis
  
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KeyWord:Benincasa hispida Cogn. seed oil  ultrasound  extraction  fatty acid composition
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ZHOU Junmei ，ZHANG Hailong，LEI Shuijuan，et al  
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Abstract:
      The ultrasound-assisted extraction of Benincasa hispida Cogn. seed oil was studied. The extraction conditions were optimized by orthogonal experiment based on single factor experiment, and the fatty acid composition of Benincasa hispida Cogn. seed oil was analyzed by GC-MS. The results showed that the optimal extraction conditions were obtained as follows: with n-hexane as solvent, ratio of solid to liquid 1∶5, ultrasonic power 400 W, extraction temperature 60℃ and ultrasonic time 10 min. Under the optimal conditions, the extraction rate was up to 96.56%. Seven kinds of fatty acids were identified from Benincasa hispida Cogn. seed oil, and they were palmitic acid (30.78%), palmitoleic acid(0.15%), heptadecanoic acid(0.21%), oleic acid(5.45%), linoleic acid (48.55%), stearic acid (14.31%) and arachidic acid(0.55%). The water content and acid value of Benincasa hispida Cogn. seed oil were 0.13% and 2.16 mgKOH/g respectively, and the color was bright orange.
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