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| Aqueous enzymatic extraction process of oil and protein from grape seeds |
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| DOI： |
| KeyWord:aqueous enzymatic method grape seed oil protein extraction amount physicochemical property |
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| Abstract: |
| In order to make full use of byproducts of grapes after brewing, the extraction processes of oil and protein from grape seeds by aqueous enzymatic method were optimized, and the effects of different drying and extraction ways on the extraction amount and physicochemical properties of grape seed oil were investigated. The results showed that the optimal extraction conditions were obtained as follows: mass ratio of cellulose, pectinase, xylanase and trypsin 2∶1∶2∶1, pH 6.5, ratio of material to liquid 1∶9 and enzymolysis time 9 h. Under these conditions, the extraction amount of grape seed oil, extraction amount and extraction rate of protein were 99.47, 74.86 mg/g and 57.58%, respectively. Different drying ways had obvious influences on the extraction amount and physicochemical properties of grape seed oil. Consideration from extraction amount of oil, quality and cost, air drying should be preferred in production. Compared with traditional methods, the quality of grape seed oil extracted by aqueous enzymatic method was better, and the protein byproducts could be obtained directly. However, the extraction efficiency was not ideal enough, which need further studies. |
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