| In order to avoid the oxidation of flaxseed oil and improve digestion-absorption in humans body, flaxseed oil nanoliposome was prepared by ethanol injection-ultrasound method. The preparation process of flaxseed oil nanoliposome was optimized by single factor experiment, and the nanoliposome was characterized, and then its in vitro release performance was studied. The optimal process conditions were obtained as follows: phosphate buffer solution pH 6.6, dosage of flaxseed oil 40% （based on total mass of soybean lecithin, β-stitosterol and Tween80）, ultrasonic time 20 min and ultrasonic power 141 W. Under these conditions, the entrapment efficiency, particle size, average potential and poly dispersity index of flaxseed oil nanoliposome were 84%，97 nm,-3.5 mV and 0.226, respectively. The particles were spherical and dispersed uniformly via the transmission electron microscopy observation. The release behaviors of flaxseed oil nanoliposome in vitro simulated gastrointestinal digestion were consistent with the zero-order kinetics equation and the Higuchi equation respectively. |
