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Preliminary study on antioxidant activity in vitro of konjac glucomannan and its oleic acid esterified product |
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KeyWord:konjac glucomannan konjac glucomannan oleic acid ester antioxidant activity |
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Abstract: |
The antioxidant activities in vitro of konjac glucomannan (KGM) and the konjac glucomannan oleic acid ester (KGM ester) were studied. By oxidation induction test, DPPH free radical scavenging, ABTS free radical scavenging, Fe2+ chelating ability and reducing power test, it was found that KGM and KGM ester had certain antioxidant activities and the higher degree of esterification, the stronger the antioxidation capacity. When dosages of KGM and KGM ester 1 with the highest esterification degree were 0.8%, the oxidation induction time of rapeseed oil extended 5.94 h and 2.56 h respectively compared with control group. In the synergy test of KGM ester and tea polyphenols, VC and TBHQ, KGM ester also showed certain antioxidant activity. When the mass concentrations of KGM and KGM esters were 0.5 mg/mL, DPPH free radical scavenging rates were 61.68% and 36.93% respectively. When the mass concentrations were 2.0 mg/mL,the ABTS free radical scavenging rates were 20.31% and 17.26%, and the Fe2+ chelating rates were 17.58% and 9.79% respectively. Although the antioxidant capacity of KGM slightly decreased after esterification with oleic acid, it still had antioxidant ability, and it was positively correlated with the degree of esterification. |
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